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Modified Harris Hematoxylin is an acidified, ready-to-use formulation that provides traditional nuclear staining results. Harris hematoxylin procedures require acid alcohol differentiation. The nuclear chromatin and cytoplasm are overstained and the acid alcohol is used to destain the cytoplasm and to remove excess stain from the nucleus. Staining times are typically in the 5-minute range. Epredia Differentiating Solution is recommended for use with Modified Harris Hematoxylin